Analytical Chemistry

IITRI's Analytical Chemistry team plays a key role in the performance of toxicology studies conducted by the Life Sciences Group. Our highly trained staff provides dose analysis, as well as stability and homogeneity analysis for solutions, suspensions, solids, diets, aerosols, vapors, and combustion products.

Additionally, we develop quantitative, validated methods for traditional, small-molecule therapeutics, as well as novel biotechnology-derived macromolecules. These methods are either developed de novo or implemented from existing procedures.

Our chemists have the experience necessary to measure test articles and metabolites in plasma, serum, whole blood, urine, and other tissues, as well as to determine metabolic profiles of various test articles.

Our expertise in protein and enzyme biochemistry can be applied to problems such as plasma protein binding, metabolism by cytochrome P450 isozymes, drug-drug interactions, in vitro percutaneous absorption, and investigating biochemical mechanisms of action. We also have experience in pharmacokinetic modeling of clinical data.

Our instrumentation, from traditional HPLC and GC to LC/MS/MS and in-line radiochemical detection, provides us with the capabilities to meet a wide range of analytical needs.

Areas of Expertise Include:

• Development and Validation of Analytical Methods to Meet Regulatory Requirements
• Characterization of Test Articles for Identity, Purity and Potency
• Homogeneity and Stability Analyses for Dosing Formulations and Diets
• Characterization and Monitoring of Aerosols, Vapors and Combustion Products for Atmospheric Exposures
• Processing Tissue and Excreta for Mass-Balance Analysis with Radio-Labeled Compounds
• Quantitation of Compounds and Metabolites in Biological Fluids and Tissue
• In Vitro Percutaneous Absorption Determinations
• In Vitro Metabolism Studies Using Microsomes, Hepatocytes, or Isolated Cytochrome P450
• Absorption, Disposition, Metabolism and Excretion Studies